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A High-Throughput System for Screening Potential
Antiviral Compounds Using the CellTiter 96® AQueous One Solution
Assay
Thomas Fletcher III, Ph.D., Roger Ptak, B.S., Stacy
Bartram, B.S., Susan Halliday, M.S. and Robert Buckheit, Jr., Ph.D.
Infectious Disease Research Department, Serquest, Southern Research Institute, Frederick,
MD; and
Rich Moravec, B.S., and Terry Riss, Ph.D, Promega Corporation
Promega's CellTiter 96® AQueous One Solution Assay features the
MTS tetrazolium compound, which is converted by viable cells into an intensely colored
formazan product that is directly soluble in tissue culture media. The reagents in the
CellTiter 96® AQueous One Solution System enable design of
homogeneous high-throughput screening assays to monitor cell viability as an
endpoint. In this article, a virus-induced cytopathic effect (CPE)-inhibition assay using
Promega's CellTiter® 96 AQueous One Solution Assay is used to
evaluate compounds for antivial activity against a variety of viruses.
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