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Technically Speaking: Ribo m⁷G Cap Analog

Abigail Farfan, M.A.
Promega Corporation

Most eukaryotic mRNAs contain an m⁷G(5´)ppp(5´)G cap at the 5´ end, which is important for the binding of translation initiation factors such as eIF4E, for RNA processing and transport, and for protection against cellular nucleases. The capped RNA can be used for in vitro translation with lysates, with Xenopus oocytes, and for processing and in vitro splicing in nuclear extracts. This Technically Speaking article answers questions on the role of the new Ribo m⁷G Cap Analog for in vitro transcription and translation.

Promega Notes 74 (2000) 23–24: Request this issue.
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