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Design of Homogeneous Luciferase Assays
Keith V. Wood, Ph.D.
Promega Corporation
Homogeneous luciferase assays can provide quantitative analysis of luciferase
expression with more speed and less effort than traditional luciferase assays. However,
the design of homogeneous assays can substantially affect data quality and quantitative
robustness. Of greatest concern is achieving uniform luminescence potential within the
sample wells. This can be compromised by concentration-dependence of the assay reagent and
by formation of luminescence gradients when the reagent is combined with cell culture
media. The formulation of Promega's Steady-Glo™ Reagent contains innovative
features that overcome these limitations.
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