Using the Access RT-PCR System: Reaction Parameters That Affect
Efficient Amplification
By Daniel Kephart, Ph.D.
Promega Corporation
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The analysis of RNA using amplification-based methodologies is a fundamental
technique used in many research and diagnostic applications. While the isolation of
high-quality RNA is fundamental to the success of RT-PCR, a variety of reaction parameters
can profoundly affect efficient amplification of target sequences. This article
demonstrates how titration of several amplification reaction components can affect
amplification of endogenous RNA targets using the Access RT-PCR System.
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