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Isolation and Analysis of Nucleic Acids from Human Blood:
A Molecular Diagnostics Application
By Daniel Kephart, Ph.D., and Hemanth Shenoi, Ph.D.
Promega Corporation
Rapid processing of blood samples is critical for maximizing the yield and quality of
isolated nucleic acids. However, the appropriate processing of blood samples may be
impractical or impossible with samples such as archived blood. This report demonstrates
the successful isolation of total RNA from frozen whole blood using Promega's SV Total RNA
Isolation System. However, when compared to blood treated to remove nonleukocyte material
prior to freezing, the unprocessed, frozen whole blood yields less RNA and RNA that does
not perform as well in RT-PCR .
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