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TransFastTM Transfection
Reagent Update
By Jennifer Oler, B.S., and Elaine Schenborn, Ph.D.
Promega Corporation
TransFastTM Transfection Reagent is comprised of the synthetic cationic lipid, N,N [bis
(2-hydroxyethyl)-N-methyl-N-[2,3-di(tetradecanoyloxy) propyl] ammonium iodide, and the
neutral lipid, DOPE. The TransFastTM Reagent is supplied as a dried
lipid film that forms multilamellar vesicles upon hydration with water. The experiments
described in this article address some commonly asked questions about the protocol for use
of this reagent.
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