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Postmortem Isolation and
Analysis of Human Genomic DNA from HIV-Infected Tissues: A Molecular Diagnostics
Application
By Brant Burkhardt, M.S., Carter Coberley, B.S., John Sleasman,
M.D., and Maureen Goodenow, Ph.D., University of Florida
Dan Kephart, Ph.D., Promega Corporation
The rapid isolation and analysis of human genomic DNA is of
fundamental importance in a variety of research procedures, both forensic and diagnostic.
Isolation of genomic DNA is often complicated by the type and size of the tissue sample,
handling and storage, potentially infectious agents in the tissue and time constraints.
This article describes the isolation of high-quality genomic DNA from human
immunodeficiency virus (HIV)-infected tissue samples using a modification of the SV Total
RNA Isolation System. The technique is
particularly well-suited to infectious material processing, where mechanical lysis and
generation of aerosols must be avoided. This method is rapid, efficient and flexible, and
produces high-quality genomic DNA for subsequent techniques such as PCR amplification.
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