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Rapid Isolation of Genomic
DNA from Human Samples: Forensics and Molecular Diagnostics Applications
By Dan Kephart, Ph.D.
Promega Corporation
Many molecular biological techniques require genomic DNA as a
starting material for manipulation. This is especially true for the molecular diagnostic
and forensic disciplines, where the rapid and robust isolation of small quantities of
human tissues is critical. This report demonstrates an extremely simple and rapid
modification of the versatile SV Total RNA Isolation System
that enables the isolation of genomic DNA from a variety of fresh and dried human source
samples in as little as 15 minutes. The DNA isolated using this technique is suitable for
analysis by standard analytical techniques such as PCR.
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