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Isolation of RNA from Pacific Oyster Larvae Using
the SV Total RNA Isolation System
By Susan Steele, Ph.D., and Michael C. Thorndyke, Ph.D.,
Biological Sciences, Royal Holloway, University of London, UK
David C. Wilkes, Ph.D., and Geoffrey Goldspink, Ph.D., Department of Anatomy, Royal Free
Hospital, London, UK
The SV Total RNA Isolation System was
used as part of a trial to determine an efficient method for RNA extraction from larvae of
the Pacific oyster, Crassostrea gigas. Due to the small size of the larvae, their
shells cannot be removed by dissection prior to nucleic acid purification. This report
details the successful use of the SV Total RNA Isolation System for purification of RNA
from these larvae and investigates the amount of larval tissue needed for RNA isolation
with the system. Purity and yield of the isolated RNA is determined by spectrophotometric
analysis.
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