|
Download
article
197kb
pdf? |
|
Wizard® Plus
Minipreps for the Isolation of
Binary Plasmids from Agrobacterium tumefaciens
By Ross A. Rupert, M.S., and Paul J. Bottino, Ph.D.
Department of Cell Biology and Molecular Genetics, University of Maryland at College Park
Minor modification of the Wizard® Plus Minipreps
DNA Purification System procedure allows for the
efficient isolation of binary vector DNA from Agrobacterium tumefaciens. Following
purification, plasmid DNA can be digested with restriction endonucleases and analyzed by
gel electrophoresis. The modified procedure serves as an effective method for screening
A.
tumefaciens cells for the presence of introduced binary vectors.
|
