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Recombinant Human Mast Cell Tryptase
By Andrew L. Niles, M.S., Mark Maffitt, M.S.,
David A. Johnson, Ph.D., Randy L. Hoffman, B.S., and Mary
Haak-Frendscho, Ph.D.
Promega Corporation and Department of Biochemistry and Molecular
Biology, East Tennessee State University
Promega has developed an expression system for generating
enzymatically active recombinant human mast cell tryptase (rhTryptase).
Available only from Promega, this enzyme is produced in Pichia pastoris and
purified by affinity chromatography. Anti-Human Tryptase mAb Biotin also is available from
Promega for use in immunostaining, Western analysis and ELISA.
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