|
Download
article
284kb
pdf? |
|
Separate
Isolation of Genomic DNA and Total RNA from Single Samples Using the SV Total RNA
Isolation System
By Paul Otto, Dan Kephart and Rex
Bitner, Promega Corporation; Suzanne Huber and Karl Volkerding, Department of Pathology
and Laboratory Medicine, University of Wisconsin-Madison
Promega's SV Total RNA Isolation System provides a fast and simple technique for the purification of
intact total RNA from tissues, cultured cells and whole blood samples (1). We describe
here a modification of the protocol that provides a rapid and safe method for the
sequential purification of DNA and RNA from the same sample of tissue, cultured cells or
whole blood, using Promega's SV Total RNA Isolation System. A simple DNase step minimizes
genomic DNA carryover from the purified RNA when only RNA is desired. The method also
allows the purification of only genomic DNA, when this is desired. The nucleic acids
isolated using this method are of high purity and are ideally suited for applications such
as cDNA cloning, RT-PCR, PCR, Northern and Southern blots and RNase protection assays.
With a minor modification, this protocol may also be used for the isolation of hepatitis C
virus (HCV) RNA suitable for RT-PCR.
|
