|
Download
article
198kb
pdf? |
|
Isolating Nucleic Acids from Small Amounts of Brain Lysates Using
the SV Total RNA Isolation System
By Dan Kephart and Kyle Hooper
Promega Corporation
The isolation of RNA from tissue and cellular
sources is a necessary first step in a diverse array of molecular biological techniques.
This report investigates the use of Promega's SV Total RNA Isolation System (Cat.# Z3100) with decreasing amounts of rat total brain
lysates. The amount of RNA isolated was linear over the range of tissue tested.
Spectrophotometric analysis and the amplification of specific neural targets demonstrate
the excellent quality and performance of the isolated RNA.
|
