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New Anti-ACTIVETM
MAPK & 'pan ERK 1/2' Antibodies for Western Analysis
By Bruce W. Jarvis and Rich Moravec
Promega Corporation
Since 1996, Promega has offered a rabbit
polyclonal antibody (pAb) that recognizes the dually phosphorylated, active form of
Extracellular signal-Regulated protein Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK).
This Anti-ACTIVETM MAPK pAb has been reformulated in a smaller size and
for use at a lower dilution. Both the original and the new Anti-ACTIVETM
MAPK pAb are affinity-purified, rabbit polyclonal antibodies capable of recognizing the
dually phosphorylated pTEpY motif in the recombinant enzyme and in the native enzyme found
in activated cell extracts. Additionally, we now offer a new Anti-ERK 1/2 pAb (pan ERK 1/2
pAb) that binds to isoforms 1 and 2 of both active and inactive ERK/MAPK. These two new
antibodies are described in the context of Western analysis.
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