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Molecular
Diagnostics: Isolation and Analysis of RNA
from Human Blood
By Dan Kephart and Hemanth Shenoi
Promega Corporation
The isolation of nucleic acids from biological
sources and subsequent analysis using amplification-based methodologies comprise an
extremely powerful approach used in many research and diagnostic applications. Blood is an
attractive sample source for nucleic acid isolation since it is readily available and rich
in genetic information. Promega has developed a variety of technologies useful for
isolating RNA and DNA from blood for subsequent analysis in a variety of applications.
Blood is a complex matrix from which to isolate nucleic acids, and particular care should
be observed when using blood as a sample source. This article is an introduction to
various parameters critical for isolating high quality nucleic acids from blood and
presents data showing how the collection, treatment and storage of collected blood can
affect subsequent RNA isolation and analysis.
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