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Quantitative
RT-PCR: Rapid Construction of Templates for Competitive Amplification
By Dan Kephart
Promega Corporation
Reverse transcription coupled with PCR
(RT-PCR) is a valuable technique for detecting rare RNA species present in cells or
tissues. Methodologies that extend RT-PCR to provide quantitative information have been
described, thus coupling the extreme sensitivity of qualitative amplification with the
added ability to quantitatively measure the level of RNA expression under different
experimental conditions. In this report, we demonstrate the design and construction of a
control template that can be used for competitive RT-PCR.
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