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Assay Systems
for Detecting Apoptosis and Cell Death
By Rich Moravec and Terry Riss
Promega Corporation
Most eukaryotic cells have the ability to
self-destruct by activation of an intrinsic cellular suicide program referred to as
programmed cell death or apoptosis. Many assay systems are available for detecting
apoptosis, providing the ability to measure various parameters associated with the cell
death process. This study illustrates the utility of the CellTiter 96® AQueous
One Solution Cell Proliferation Assay, the CytoTox 96®
Non-Radioactive Cytotoxicity Assay, the CaspACETM Assay System,
Fluorometric, and the Apoptosis Detection System, Fluorescein, as methods to elucidate
apoptotic events. These four systems measure viable cell number, cell membrane damage,
caspase activation and DNA fragmentation, respectively.
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