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Pfu
DNA Polymerase: A High Fidelity Enzyme for Nucleic Acid Amplification
By Michael Slater, Susanne Selman,
Bill Mogilevsky, Harryl Ammons and Jim Hartnett
Promega Corporation
Pfu DNA Polymerase exhibits the lowest
error rate of any thermostable DNA polymerase studied (1-3). For routine PCR, where simple
detection of an amplification product or estimation of the product's size is important,
Taq
DNA polymerase is the obvious enzyme to choose. However, when the amplified product is to
be cloned, expressed or used in mutagenesis studies, Pfu DNA Polymerase is a much
better enzyme of choice for PCR. Pfu DNA Polymerase is also used in blends with Taq
DNA polymerase, or amino-terminally truncated versions of Taq DNA polymerase, to
amplify longer stretches of DNA in PCR with greater accuracy than Taq DNA
polymerase alone.
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