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CaspACETM Assay System, Fluorometric
By Gopa Mitra and Terry Riss
Promega Corporation
Promega's CaspACETM Assay System, Fluorometric,
provides reagents for measuring the activity of two cysteine aspartic acid-specific
proteases caspase-1 (ICE) and caspase-3 (CPP32). Caspase is the accepted acronym for
this class of enzymes, the "c" is intended to reflect a cysteine protease
mechanism and the "aspase" refers to their ability to cleave after aspartic acid
(1). The CaspACETM Assay System provides fluorogenic tetrapeptide
substrates and selective inhibitors, which allow highly sensitive, rapid and quantitative
measurement of these enzymes. The use of the two selective substrates and inhibitors
provided in the system allows discrimination between ICE and CPP32 enzymatic activities.
This assay may be used to measure ICE and CPP32 activities in either purified enzyme
preparations or cell extracts and can be adapted for use in high throughput assay systems.
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