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The CheckMateTM Mammalian Two-Hybrid System
By Elaine Schenborn and Lisa deBerg, Promega Corporation, and
William Brondyk, Ares Advanced Technologies
Two-hybrid systems provide powerful methods for detecting
protein:protein interactions in vivo. The two-hybrid system, originally developed
in yeast, has been adapted for use in mammalian cells. Promega recently introduced the
CheckMateTM Mammalian Two-Hybrid System, which allows protein:protein
interactions to be studied in the cell line of choice. In this article, the CheckMateTM
Mammalian Two-Hybrid System is demonstrated in HeLa, NIH3T3 and CHO mammalian cell lines,
as well as used to confirm in vivo association of the cloned Raf-1 kinase and
oncogenic Ras proteins.
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