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Technically Speaking
GeneEditorTM in vitro
Site-Directed Mutagenesis System
Pegg Kopps
Promega Corporation
The GeneEditorTM in vitro Site-Directed
Mutagenesis System* uses positive selection for antibiotic
resistance to allow high efficiency oligo-directed mutagenesis (1). During the mutagenesis
reaction, the ampicillin resistance gene is mutated to give resistance to the GeneEditorTM
Antibiotic Selection Mix. The desired mutations are also made in the same mutagenesis
reaction. Any vector containing the ampicillin resistance gene can be used. Positive
selection of mutants, such as that used in the GeneEditorTM System,
provides advantages over other site-directed mutagenesis systems in terms of efficiency
and ease of use. Here we answer some of the most frequently asked questions about
Promega's GeneEditorTM in vitro Site-Directed Mutagenesis System.
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