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Stability of pGEM®-T
Vectors
By Rich Rhodes
Promega Corporation
PCR products are frequently cloned
into plasmid vectors for subsequent manipulations, such as sequencing and mutagenesis. The
pGEM®-T Vector Systems,
and other T-vectors available from Promega, allow direct cloning of PCR products without
restriction digestion, modification of primers or purification of amplified DNA. The
thymidine overhangs of the pGEM®-T Vectors are stable even when the DNA
is subjected to freezing and heating.
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