|
Download
article
476kb
pdf? |
|
TfxTM-50 Reagent and High Efficiency Transfection
of Vascular Tissue in vitro and in vivo
By Daxin Chen, Florea Lupu, Nick Shaper, Jacqueline F.
Schmitt, Nicholas R. Lemoine and Michael-Christopher Keogh
Recent studies have demonstrated the use of cationic liposomes to
facilitate the functional delivery of a reporter gene into vascular cells in vitro
or into the vascular wall in vivo. We describe the successful delivery of a
luciferase gene into vascular cells in vitro and tissues in vivo using the
TfxTM-50 Reagent, a cationic liposome.
Our methods also resulted in higher efficiencies than previously reported for a
transfection complex of cationic liposome:DNA in vascular tissue.
|
