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in vitro Transcription on DNA
Templates Immobilized to Streptavidin MagneSphere® Paramagnetic Particles
By Mingyi Liu and David H. Price
Department of Biochemistry, University of Iowa, Iowa City, IA
The immobilization of molecules has successfully aided research in many areas,
including purification of proteins and the study of protein-protein and protein-nucleic
acid interactions. We have used Promega's Streptavidin MagneSphere®
Paramagnetic Particles (Cat.# Z5481) to immobilize DNA templates for use in the study of
RNA polymerase II complex formation and transcription in vitro. The ability to
rapidly isolate transcription complexes formed on the immobilized templates and the ease
with which reaction conditions can be manipulated are particularly helpful for dissecting
the complex interactions inherent in transcription.
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