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RNA Purification: A Rapid and Versatile
Protocol for the Isolation of Total RNA
By Paula Brisco, Jacqui Sankbeil and Dan Kephart
Promega Corporation
Promega's new SV Total RNA Isolation System offers
a complete system for the isolation of quality RNA from small amounts of starting
material. This system provides a fast and simple technique for the preparation of purified
and intact total RNA from tissues, cultured cells and whole blood samples. This
purification is achieved without the use of phenol/chloroform extractions and employs a
simple DNase step to eliminate genomic DNA efficiently. No genomic DNA contamination is
seen when the RNA is analyzed by reverse transcription-PCR (RT-PCR). In this article, we demonstrate that RNA isolated using this system is highly
pure and is ideally suitable for applications such as RT-PCR.
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