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Technically Speaking
Tips for Working with RNA and
Troubleshooting Downstream Applications
By Mark Lepinske
Promega Corporation
High quality RNA is required for many molecular biology techniques. The isolation
and use of purified RNA in the laboratory is complicated by the fact that, chemically and
biologically, RNA is significantly more labile than DNA. The choice and optimization of
RNA purification methods are important for successful isolation of quality RNA and
consistent performance of downstream applications. In order to avoid damage or loss of
RNA, caution should be exercised to avoid nuclease contamination of the RNA samples during
preparation and handling.
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