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Rapid Isolation of RNA from Small Quantities of Human Whole Blood
for use in RT-PCR
Analysis
By Daniel Kephart
Promega Corporation
Isolation of RNA from whole blood and analysis by RT-PCR is a
powerful technique that is useful for a variety of applications. We have used Promega's
RNAgents® Total RNA Isolation System, the PolyATtract® System
1000, and a modified freeze-thaw technique to develop RNA isolation protocols that use
small amounts (less than 100µl) of human whole blood as starting material. The RNA
samples obtained were analyzed using the Access RT-PCR System to
specifically detect the presence of an endogenous human RNA species. These techniques
enable the rapid and efficient isolation of RNA from whole blood and could readily be
scaled-up to a high-throughput format.
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