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The GeneEditorTM in vitro Mutagenesis System:
Site-Directed Mutagenesis Using Altered Beta-Lactamase Specificity
By Christine Andrews and Scott Lesley
Promega Corporation
Promega's new GeneEditorTM in vitro
Site-Directed Mutagenesis System* provides a high efficiency, reliable
procedure for the generation and selection of oligonucleotide-directed mutants. The
Selection Oligonucleotides provided in this system encode mutations which alter the
substrate specificity of beta-lactamase, the gene encoding ampicillin resistance, commonly
found on cloning vectors. This altered substrate specificity provides a positive selection
for the mutant strand and results in consistently high mutagenesis frequencies using
either double-stranded (ds) or single-stranded (ss) DNA templates.
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