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Technically Speaking
RNAgents® Total RNA Isolation System
By Douglas Barron
Promega Corporation
Promega's RNAgents® Total RNA Isolation System is
designed for the rapid and convenient isolation of total RNA from animal tissue, plant
tissue and cultured cells. The system has been modified to increase ease of use,
performance in total RNA recovery and flexibility with starting source of RNA. The purity
and integrity of RNA isolated from tissue or cultured cells are critical for its effective
use in a wide array of downstream applications. In recent years, reverse transcription
PCR (RT-PCR) has emerged as a powerful method to identify and quantitate tissue-specific
rare mRNAs from small amounts of total RNA and mRNA. In addition, RT-PCR allows for the
cloning of cDNA products without the need for constructing and screening cDNA libraries.
As the use of amplification as a research tool has grown, the need for methods to rapidly
isolate high quality RNA from small amounts of starting material (i.e., tissue and
cultured cells) has also increased. The RNAgents® Total RNA Isolation System
has been redesigned to address these needs.
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