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Amplification of Flanking Regions: New Applications and Performance
Optimization of Single Specific Primer PCR and T-Vector Cloning
By Jan Novak and Lea Novak
University of Alabama at Birmingham
Single specific primer PCR(a) (SSP-PCR), designed
for DNA amplification when only one gene-specific primer is available, has been shown to
amplify fragments of up to 1.8kb. By optimizing this technique, we have been able to
amplify significantly longer fragments. We have utilized this strategy in a variety of
cloning applications. Here, we describe optimization of the SSP-PCR technique for the
generation of long, specific amplification products.
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