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AgarACETM Agarose-Digesting
Enzyme Part Three: DNA/RNA
Recovery and Applications Testing for Use in Translation, Transcription/Translation,
Prime-a-Gene® Labeling and Fluorescent DNA Sequencing
By Mark Knuth, Kimberly Knoche and Paul Otto
Promega Corporation
AgarACETM Enzyme is a unique agarase that
enables simple, quantitative recovery of nucleic acids from agarose gels. Previously, we
presented data describing the use of AgarACETM Enzyme in cloning,
sequencing and PCR-related applications (1,2). In this article, we present data on
recovery of supercoiled plasmid DNA, high molecular weight linear DNA and RNA, and show
that DNA and RNA isolated using AgarACETM Enzyme perform well in four
additional popular applications.
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