Site-Directed Mutagenesis
By Neal Cosby and Scott Lesley
Promega Corporation
Site-directed mutagenesis: "Any of various techniques by
which defined mutations can be made in vitro in a cloned DNA (1)."
Site-directed mutagenesis has become one of the most commonly
used methods in molecular biology. Here we trace its origins, provide information about
resources that are available to facilitate oligonucleotide design and provide a brief
analysis of some of the commonly used mutagenesis approaches.
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