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A Comparative Study of T7 RNA Polymerase Quality
By Kimberly Knoche, Jude Stevens and Ray Bandziulis
Promega Corporation
Many vendors supply bacteriophage RNA polymerases for in
vitro transcription applications; however, the unit activity assays used by these
vendors vary widely. In order to test the consistency and reproducibility of the results
obtained from different enzyme sources, we compared T7 RNA polymerases from six leading
vendors. We measured unit activity and performed standard quality assurance tests. In this
article, we present the results of our comparisons.
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