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In vitro Transcription from Biotinylated DNA Immobilized on Streptavidin
MagneSphere® Paramagnetic Particles
By Gary Kobs
Promega Corporation
Rapid purification and in vitro transcription of PCR
products can be accomplished using Promega's Streptavidin MagneSphere®
Paramagnetic Particles and RiboMAXTM RNA Production Systems(a). The
technique described in this update yields optimal amounts of high quality RNA, which can
be used for further applications such as RNA probes or in vitro translation.
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