|
Download
article
262kb
pdf? |
|
Improved PCR Amplification Using TaqBeadTM
Hot Start Polymerase
By Katherine Miller, Rick Smith and Doug Storts
Promega Corporation
TaqBeadTM Hot Start Polymerase(a)
offers a simple method for increasing the yield and specificity of PCR(b)
amplification. Template, primers, buffer, magnesium and dNTPs are added to each tube at
room temperature, either individually or from a reaction cocktail. A single TaqBeadTM
Hot Start Polymerase bead(c), containing Taq DNA
Polymerase(a), is added to each reaction tube. Upon heating the sample
to 60°C, the wax bead melts, liberates the enzyme and allows the reaction to proceed. The
use of TaqBeadTM Hot Start Polymerase significantly reduces
the amount of primer-dimer and nonspecific amplification products that often are
associated with "cold start" amplification methods.
|
