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BSA and Restriction Enzyme Digestions
By Ray Williams, Marty Kline and Rick Smith
Promega Corporation
Recently we have developed performance assays comparing the
effect of the addition of acetylated bovine serum albumin (BSA) to restriction enzyme
digestions. We compared the effects of adding BSA to restriction enzyme digests performed
using enzymes from several different suppliers, and using DNA purified using two different
techniques. We have also examined the effect of the presence of BSA on restriction enzyme
digestions performed using different reaction vessels. In nearly all instances, the
presence of BSA enhanced the performance of restriction enzymes by at least two-fold.
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