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A Trio of TfxTM Transfection Reagents for Eukaryotic
Cells
By Elaine Schenborn, Jennifer Oler and Virginia Goiffon
Promega Corporation
Promega's TfxTM Reagent(a) product line
has recently been expanded to include the TfxTM-10, TfxTM-20 and TfxTM-50
Reagents. All these reagents contain the same cationic lipid species, but are formulated
with different molar ratios of the neutral lipid component, L-dioleoyl
phosphatidylethanolamine (DOPE). Differences in the proportion of cationic:neutral lipid
result in changes in the transfection efficiency for different cell types. The TfxTM-10,
TfxTM-20 and TfxTM-50 Reagent formulations thus provide a range of
transfection reagents which give optimal performance in multiple cell lines. To facilitate
selection of the optimal TfxTM Reagent for your cell lines, all three of these
formulations are available in the new TfxTM Reagents Transfection Trio.
(a)The cationic lipid component of the TfxTM Reagents
is covered by U.S. Pat. No. 5,527,928 and pending foreign patents.
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