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Detection and Quantitation of Protein Tyrosine Kinases
By Erik Schaefer and Kevin Hsaio, Promega Corporation and
Scott Guimond, Department of Pathology, University of Wisconsin
Widespread appreciation of the complexity of signaling
pathways involving the protein tyrosine kinase (PTK) superfamily of enzymes has resulted
in the need for more sophisticated reagents to dissect the role of PTKs in normal and
disease states. To meet this need, Promega has developed the SignaTECTTM
PTK Assay System, a novel method based on the use of optimized PTK Biotinylated Peptide
Substrates in conjunction with the unique SAM2TM Biotin Capture
Membrane. This system provides selective, accurate and rapid detection of PTK activity,
while minimizing handling and radioisotope requirements. The SignaTECTTM
PTK Assay System is a broad-specificity assay that measures the activity of a wide variety
of PTKs, including both transmembrane receptor and cytosolic enzymes.
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