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Technically
Speaking
Access RT-PCR System
By Patrick Burke
Promega Corporation
Transcription of RNA from DNA is the principal step in
executing a cell's genetic program. Numerous techniques have been developed to investigate
gene expression at the level of transcription, including Northern blot hybridization,
RNase protection and reverse transcription coupled with polymerase chain reaction
amplification (RT-PCR). RT-PCR is a sensitive and versatile method which can be used to
determine the presence of a transcript, to estimate expression levels and to clone cDNA
products without the necessity of constructing and screening a cDNA library. Promega's
Access RT-PCR System(a) provides a convenient, single-tube, two-enzyme method
for generating a cDNA product from an RNA sample.
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