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The pGEM®-T and pGEM®-T Easy Vector
Systems
By Liz Marcus, Jim Hartnett and Douglas R. Storts
Promega Corporation
The use of T vectors can greatly facilitate the cloning of PCR
products. In this update, we describe a new version of the pGEM®*-T Vector
available in the pGEM®-T Easy Vector Systems. The pGEM®-T Easy
Vector is produced by modifying the multiple cloning site (MCS) of the original pGEM®-T
Vector so that a single restriction digestion with either Not I or EcoR I
may be performed to remove the insert DNA. We also describe ways to optimize the results
obtained using the pGEM®-T and pGEM®-T Easy Vector Systems.
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