|
Download
article
209kb
pdf? |
|
The TNT® T7 Quick Coupled
Transcription/Translation System
By Robin Hurst, Mark Maffitt, Elizabeth Murray, James Kappelman, Qin-yu Xu, Jacqui
Mendez, Braeden Butler, Angela Ryan and Gregory S. Beckler
Promega Corporation
The TNT®* T7 Quick Coupled
Transcription/Translation System offers researchers a convenient new alternative for
eukaryotic in vitro translation -- a one tube, coupled transcription/translation
reaction. This new addition to the TNT® Coupled
Transcription/Translation Systems simplifies the process by including all of the reaction
components in a single TNT® T7 Quick Master Mix. To use this
system, 0.2-2.0µg of either circular or linear DNA is added to an aliquot of the Master
Mix and incubated in a 50µl reaction volume for 60-90 minutes at 30°C. Synthesized
proteins are then analyzed by SDS-PAGE and autoradiography or by functional activity.
|
