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Dual-Luciferase™ Reporter Assay: An Advanced Co-Reporter Technology
Integrating Firefly and Renilla Luciferase Assays
By Bruce A. Sherf, Shauna L. Navarro,
Rita R. Hannah and Keith V. Wood
Promega Corporation
In the quantitation of gene expression using firefly
luciferase, a second reporter gene is commonly used to minimize experimental variability.
However, traditional co-reporters (e.g., CAT, beta-Gal or GUS) are inconvenient due to
differences in their respective assay chemistries, handling requirements and measurement
characteristics. Promega introduces a superior dual-reporter technology integrating the
assay of firefly luciferase with the Renilla luciferase assay. The Dual-Luciferase™
Reporter Assay System, in combination with the pRL Vectors which express Renilla
luciferase as the second reporter, offers the exceptional speed, sensitivity and
convenience of two luciferase reporter assays in a single-tube format. The system also
includes Passive Lysis Buffer, formulated to provide quantitative solubilization of both
luciferases from mammalian cells cultured in multi-well plates without the need for
individual processing of each sample. The Dual-Luciferase™ Reporter
Assay System will provide immediate benefit to researchers currently using any
experimental firefly luciferase reporter vector.
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