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Tfx™-50 Reagent Increases the Uptake of Oligonucleotides by Leukemic Cells
By Gilles Labroille, Sabine Bonnefille and Francis Belloc
Laboratoire d'Hématologie, Hopital du Haut Lévèque,
Avenue Magellan, 33604 PESSAC, France
The cellular uptake of small, linear oligonucleotides is a
critical process in antisense methods for gene inactivation. Internalization of these
exogenous molecules appears to involve endocytotic vesicles and cationic lipid molecules
are known to facilitate the internalization process. Among cationic lipid molecules,
Promega's Tfx™-50 Reagent for the transfection of eukaryotic cells provides
a powerful tool to drive the delivery of linear oligonucleotides into mammalian cells. In
this article, we compare the efficiency of this product to another commercially available
lipid carrier in the delivery of a fluorescently labeled 27-mer oligonucleotide, targeted
to the multidrug-resistance gene mdr-1, in three leukemic human cell lines.
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