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A Rapid Protocol for the Isolation of Total RNA Suitable for RT-PCR
By Paula Brisco, Brian Andersen and Craig Smith
Promega Corporation
The ability to amplify and detect target sequences in RNA by the method of RT-PCR
has changed the dynamics of RNA purification for the research laboratory. Prior to RT-PCR,
methods for cloning and detecting RNA by Northern analysis required the isolation of RNA
from large quantities of tissue or cultured cells. We describe a modification to Promega's
RNAgents® Total RNA Isolation System protocol, which allows for the rapid and convenient
small-scale isolation of total RNA, suitable for applications such as RT-PCR.
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