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Isolation of Total RNA and mRNA from Plant Tissues
By: Isabel Murillo, Dora Raventos, Estelle Jaeck, Blanca San Segundo*
Centro de Investigacion y Desarrollo de Barcelona (CSIC),
Departamento de Genetica Molecular
Jordi Girona 18, 08034, Barcelona, SPAIN
*Corresponding author: Tel: 34-3-4006128 Fax: 34-3-2045904
The Promega RNAgents® Total RNA Isolation System and PolyATtract® Systems have
been used to obtain total RNA and mRNA, respectively, from maize tissues. Modifications of
the protocols provided by Promega have been made in order to adapt them to use with the
different plant tissues studied in this work. Furthermore, the PolyATtract® System 1000
has been used for the direct purification of mRNA from leaves and germinating embryos.
Yields obtained using these systems were better than standard isolation procedures. The
RNAs were of high quality and produced strong hybridization signals in Northern blot
analysis. The mRNA has been used successfully for construction of a cDNA library from
germinating maize embryos.
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