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Effects of Bacterial Strains Carrying the endA1 Genotype on DNA Quality
Isolated with Wizard™ Plasmid Purification Systems
Tom Schoenfeld, Jacqui Mendez, Douglas R. Storts, Evelyn Portman, Bill Patterson,
Judy Frederiksen and Craig Smith
Promega Corporation
We investigated the instability of plasmid DNA isolated from endA positive
E.
coli strains using a number of commercially available DNA purification systems,
including the Wizard(TM) Minipreps DNA Purification System. This phenomenon was observed
with several of the systems tested when purifying plasmid DNA from endA positive
hosts; no stability problems were observed with plasmid isolated from endA negative
host strains. Preliminary data suggests that the presence of endonuclease I, the product
of the endA gene, contributes to this inconsistent quality. We describe ways to
control this phenomenon through bacterial strain selection, growth media selection, or
slight modifications to the protocol supplied with the Wizard(TM) Minipreps DNA
Purification System.
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