|
Download
article
260kb
pdf? |
|
PCR ACCESS! A Sensitive Single-tube, Two-enzyme System for RT-PCR
by Katharine Miller and Douglas R. Storts
Promega Corporation
The relative advantages of three RT-PCR methods are discussed, including detection
sensitivity, reverse transcription temperature, fidelity and ease-of-use. The mixture of
AMV and Tfl DNA Polymerase in Promega's Access RT-PCR System gives detection
sensitivity equivalent to or better than the combination of MuLV and Taq DNA
polymerases. Both viral reverse transcriptase-based systems were significantly more
sensitive for amplification from total RNA than the rTth-based single-enzyme
system.
|
