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Using Promega's CytoTox 96® Non-Radioactive Cytotoxicity Assay to Measure Cell Death
Mediated by NMDA Receptor Subunits
Miroslav Cik, Paul L. Chazot, Sarah K. Coleman and F. Anne Stephenson
Pharmaceutical Chemistry Department,
The School of Pharmacy, University of London,
29/39 Brunswick Square, London WC1N 1AX
In this brief report we describe the use of Promega's CytoTox 96® Non-Radioactive
Cytotoxicity Assay to accurately and rapidly measure cell death mediated by transient
expression of NMDA receptor subunits following calcium phosphate transfection of mammalian
cells. This convenient method assesses cell death using medium alone and allows
biochemical analysis of recombinant receptors on the same cells used for cytotoxicity
measurements.
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