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Altered Sites® II In Vitro Mutagenesis Systems
Karen Singer
Promega Corporation
Promega recently revised and simplified the Altered Sites® II Mutagenesis Systems.
These second generation systems feature several improvements over the original Altered
Sites® Systems: use double-stranded DNA templates for mutagenesis reactions, perform
sequential rounds of mutagenesis without subcloning, and express the mutated gene product
in
vivo or in vitro. Here are answers to some of the most frequently asked
questions about Promega's Altered Sites® II Mutagenesis Systems.
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