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LPS-Stimulated MCAF Production by Bovine Parenchyma In Vitro
Daniel Mentone-Saban and Richard Broadstone
Department of Veterinary Medicine
University of Wisconsin-Madison
Madison, WI 53706
Mary Haak-Frendscho
Promega Corporation
An in vitro model of pulmonary pasteurellosis was used to examine the effect
of leukotoxin (LTX) and lipopolysaccharide (LPS) treatment on subsequent MCAF expression
in bovine lung parenchyma. Following treatment, frozen tissue sections were stained using
Promega's Anti-Human MCAF Polyclonal Antibody. The results suggest that LPS, but not LTX,
causes an up-regulation of MCAF expression and demonstrate the effectiveness of this
antibody for studying MCAF regulation.
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